Review



claudin 4  (Proteintech)


Bioz Verified Symbol Proteintech is a verified supplier  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
    • 99
      Buy from Supplier

    Structured Review

    Proteintech claudin 4
    Claudin 4, supplied by Proteintech, used in various techniques. Bioz Stars score: 99/100, based on 121 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/claudin+4/CLDN7+Fusion+Protein/pm41932000-61-5-13
    Average 99 stars, based on 121 article reviews
    claudin 4 - by Bioz Stars, 2026-10
    99/100 stars

    Images

    Related Articles

    Incubation:

    Article Title: Curcumin ameliorates aging-induced blood-testis barrier disruption by regulating AMPK/mTOR mediated autophagy.
    Article Snippet: .. The membranes were then incubated overnight at 4 °C with specific primary antibodies: P-AMPK alphaThr172 (2535, 1:1000, Cell Signaling Technology, Danvers, Massachusetts, USA), AMPK alpha (5831s, 1:1000, Cell Signaling Technology), LC3B (ab192890, 1:2000, Abcam, Cambridge, MA, USA), Beclin1 (bs-1353R, 1:500, Bioss), P-mTOR (T56571, 1:1000, Abmart, Shanghai, China), mTOR (T55306, 1:1000,Abmart), p21 (T55543S, 1:1000, Abmart), CDKN2A (TN23895S, 1:1000, Ab-mart), Occludin (27260–1-AP, 1:1000, Proteintech, Wuhan, China), Claudin-4 (16195–1-AP, 1:1000, Proteintech), Claudin-7 (29795–1-AP, 1:1000, Proteintech), ZO-1 (21773–1-AP, 1:1000, Proteintech), β-Actin (bs-0061R, 1:3000, Bioss). .. Next, the membranes were washed and incubated with HRP-conjugated Goat Anti-Rabbit IgG (Proteintech, SA00001–2, 1:5000)/ HRP-conjugated Goat Anti-Mouse IgG (Proteintech, SA00001–1, 1:5000) for 1 h at 37 °C.

    Article Title: Pharmacokinetic and Pharmacodynamic Evaluation of PZ-2891, an Anti-Alzheimer’s Disease Agonist of PANK2
    Article Snippet: .. After transferring to a PVDF membrane and blocking in 5% BSA or skim milk for 2 h, blots were incubated with primary antibodies at 4 °C overnight, namely GAPDH (1:40,000, Abcam EPR16891 ), p-tau396 (1:500, CST F3S9T), Aβ (1:1000, Proteintech 25524-1-AP), GFAP (1:10,000, Proteintech 16825-1-AP), LRP1 (1:1000, Proteintech 26106-1-AP), MRP1 (1:1000, Proteintech 67228-1-Ig), PANK1 (1:1000, Proteintech 11768-1-AP), PANK2 (1:1000, Proteintech 11001-1-AP), PANK3 (1:500, SCBT sc-551231), occludin (1:2000, Proteintech 27260-1-AP), ZO-1 (1:1000, Proteintech 21773-1-AP), claudin-1 (1:1000, Proteintech 28674-1-AP) and claudin-4 (1:1000, Proteintech 16195-1-AP). .. After incubating with secondary antibodies (1:10,000 Bioworld BS13278) for 2 h, the target proteins were detected in a ChemDocTM XRS+ gel imaging system via the enhanced chemiluminescence (ECL) method.

    Article Title: Curcumin ameliorates aging-induced blood-testis barrier disruption by regulating AMPK/mTOR mediated autophagy
    Article Snippet: .. The membranes were then incubated overnight at 4 °C with specific primary antibodies: P-AMPK alphaThr172 (2535, 1:1000, Cell Signaling Technology, Danvers, Massachusetts, USA), AMPK alpha (5831s, 1:1000, Cell Signaling Technology), LC3B (ab192890, 1:2000, Abcam, Cambridge, MA, USA), Beclin1 (bs-1353R, 1:500, Bioss), P-mTOR (T56571, 1:1000, Abmart, Shanghai, China), mTOR (T55306, 1:1000,Abmart), p21 (T55543S, 1:1000, Abmart), CDKN2A (TN23895S, 1:1000, Ab-mart), Occludin (27260–1-AP, 1:1000, Proteintech, Wuhan, China), Claudin-4 (16195–1-AP, 1:1000, Proteintech), Claudin-7 (29795–1-AP, 1:1000, Proteintech), ZO-1 (21773–1-AP, 1:1000, Proteintech), β-Actin (bs-0061R, 1:3000, Bioss). .. Next, the membranes were washed and incubated with HRP-conjugated Goat Anti-Rabbit IgG (Proteintech, SA00001–2, 1:5000)/ HRP-conjugated Goat Anti-Mouse IgG (Proteintech, SA00001–1, 1:5000) for 1 h at 37 °C.

    Expressing:

    Article Title: Relaxin-2-secreting CAR-T cells exhibit enhanced efficacy in stromal-rich xenograft tumors.
    Article Snippet: The expression of CD44v6 in various cancer cell lines was assessed using a mouse anti-human FITCconjugated CD44v6 antibody (clone VFF-18, eBioscience). .. The expression of claudin-4 in SU86.86 and H522 was assessed using a rabbit anti-human claudin-4 antibody (cat no. 16195-1-AP, Proteintech). .. Memory/effector phenotypes of CAR-T cells were monitored using CD45RO, CD62L, and CD3 antibodies, whereas degranulation and activation were detected using CD107a, CD69, and CD8a antibodies and the Golgi blocker monensin (1,000x; BioLegend).

    Article Title: Relaxin-2-secreting CAR-T cells exhibit enhanced efficacy in stromal-rich xenograft tumors
    Article Snippet: The expression of CD44v6 in various cancer cell lines was assessed using a mouse anti-human FITC-conjugated CD44v6 antibody (clone VFF-18, eBioscience). .. The expression of claudin-4 in SU86.86 and H522 was assessed using a rabbit anti-human claudin-4 antibody (cat no. 16195-1-AP, Proteintech). .. Memory/effector phenotypes of CAR-T cells were monitored using CD45RO, CD62L, and CD3 antibodies, whereas degranulation and activation were detected using CD107a, CD69, and CD8a antibodies and the Golgi blocker monensin (1,000x; BioLegend).

    Transferring:

    Article Title: Pharmacokinetic and Pharmacodynamic Evaluation of PZ-2891, an Anti-Alzheimer’s Disease Agonist of PANK2
    Article Snippet: .. After transferring to a PVDF membrane and blocking in 5% BSA or skim milk for 2 h, blots were incubated with primary antibodies at 4 °C overnight, namely GAPDH (1:40,000, Abcam EPR16891 ), p-tau396 (1:500, CST F3S9T), Aβ (1:1000, Proteintech 25524-1-AP), GFAP (1:10,000, Proteintech 16825-1-AP), LRP1 (1:1000, Proteintech 26106-1-AP), MRP1 (1:1000, Proteintech 67228-1-Ig), PANK1 (1:1000, Proteintech 11768-1-AP), PANK2 (1:1000, Proteintech 11001-1-AP), PANK3 (1:500, SCBT sc-551231), occludin (1:2000, Proteintech 27260-1-AP), ZO-1 (1:1000, Proteintech 21773-1-AP), claudin-1 (1:1000, Proteintech 28674-1-AP) and claudin-4 (1:1000, Proteintech 16195-1-AP). .. After incubating with secondary antibodies (1:10,000 Bioworld BS13278) for 2 h, the target proteins were detected in a ChemDocTM XRS+ gel imaging system via the enhanced chemiluminescence (ECL) method.

    Membrane:

    Article Title: Pharmacokinetic and Pharmacodynamic Evaluation of PZ-2891, an Anti-Alzheimer’s Disease Agonist of PANK2
    Article Snippet: .. After transferring to a PVDF membrane and blocking in 5% BSA or skim milk for 2 h, blots were incubated with primary antibodies at 4 °C overnight, namely GAPDH (1:40,000, Abcam EPR16891 ), p-tau396 (1:500, CST F3S9T), Aβ (1:1000, Proteintech 25524-1-AP), GFAP (1:10,000, Proteintech 16825-1-AP), LRP1 (1:1000, Proteintech 26106-1-AP), MRP1 (1:1000, Proteintech 67228-1-Ig), PANK1 (1:1000, Proteintech 11768-1-AP), PANK2 (1:1000, Proteintech 11001-1-AP), PANK3 (1:500, SCBT sc-551231), occludin (1:2000, Proteintech 27260-1-AP), ZO-1 (1:1000, Proteintech 21773-1-AP), claudin-1 (1:1000, Proteintech 28674-1-AP) and claudin-4 (1:1000, Proteintech 16195-1-AP). .. After incubating with secondary antibodies (1:10,000 Bioworld BS13278) for 2 h, the target proteins were detected in a ChemDocTM XRS+ gel imaging system via the enhanced chemiluminescence (ECL) method.

    Blocking Assay:

    Article Title: Pharmacokinetic and Pharmacodynamic Evaluation of PZ-2891, an Anti-Alzheimer’s Disease Agonist of PANK2
    Article Snippet: .. After transferring to a PVDF membrane and blocking in 5% BSA or skim milk for 2 h, blots were incubated with primary antibodies at 4 °C overnight, namely GAPDH (1:40,000, Abcam EPR16891 ), p-tau396 (1:500, CST F3S9T), Aβ (1:1000, Proteintech 25524-1-AP), GFAP (1:10,000, Proteintech 16825-1-AP), LRP1 (1:1000, Proteintech 26106-1-AP), MRP1 (1:1000, Proteintech 67228-1-Ig), PANK1 (1:1000, Proteintech 11768-1-AP), PANK2 (1:1000, Proteintech 11001-1-AP), PANK3 (1:500, SCBT sc-551231), occludin (1:2000, Proteintech 27260-1-AP), ZO-1 (1:1000, Proteintech 21773-1-AP), claudin-1 (1:1000, Proteintech 28674-1-AP) and claudin-4 (1:1000, Proteintech 16195-1-AP). .. After incubating with secondary antibodies (1:10,000 Bioworld BS13278) for 2 h, the target proteins were detected in a ChemDocTM XRS+ gel imaging system via the enhanced chemiluminescence (ECL) method.

    Immunohistochemistry:

    Article Title: p38 MAPK-mediated upregulation of claudin-3 and claudin-4 by gemcitabine contributes to chemoresistance in ovarian cancer.
    Article Snippet: Chemotherapy is a primary therapeutic option in cancer treatment, but often associated with unwanted side effects and drug resistance.. Claudin-3 (CLDN3) and claudin-4 (CLDN4) are essential components of tight junctions, frequently overexpressed in ovarian cancer, serve as potential therapeutic targets.. In this study, we utilized flow cytometry, qPCR, Western blot, and animal experiments to investigate the regulation of CLDN3 and CLDN4 by chemotherapy drug, gemcitabine, in the ovarian cancer cell line A2780.

    Staining:

    Article Title: p38 MAPK-mediated upregulation of claudin-3 and claudin-4 by gemcitabine contributes to chemoresistance in ovarian cancer.
    Article Snippet: Chemotherapy is a primary therapeutic option in cancer treatment, but often associated with unwanted side effects and drug resistance.. Claudin-3 (CLDN3) and claudin-4 (CLDN4) are essential components of tight junctions, frequently overexpressed in ovarian cancer, serve as potential therapeutic targets.. In this study, we utilized flow cytometry, qPCR, Western blot, and animal experiments to investigate the regulation of CLDN3 and CLDN4 by chemotherapy drug, gemcitabine, in the ovarian cancer cell line A2780.



    Similar Products

    fitc anti human cldn4  (Miltenyi Biotec)
    91
    Miltenyi Biotec fitc anti human cldn4
    Fitc Anti Human Cldn4, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/claudin+4/Claudin-4+Antibody%2C+anti-human%2C+REAfinity/pm42277848-107-10-13
    Average 91 stars, based on 1 article reviews
    fitc anti human cldn4 - by Bioz Stars, 2026-10
    91/100 stars
    		  Buy from Supplier
    claudin 4  (Biocare Medical)
    86
    Biocare Medical claudin 4
    Claudin 4, supplied by Biocare Medical, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/claudin+4/4+anti+claudin/pm42163521-58-23-25
    Average 86 stars, based on 1 article reviews
    claudin 4 - by Bioz Stars, 2026-10
    86/100 stars
    		  Buy from Supplier
    rabbit anti cldn4  (Bioss)
    94
    Bioss rabbit anti cldn4
    Rabbit Anti Cldn4, supplied by Bioss, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/claudin+4/Claudin+4+Polyclonal+Antibody/10__1016_slash_j__psj__2026__107089-94-14-17
    Average 94 stars, based on 1 article reviews
    rabbit anti cldn4 - by Bioz Stars, 2026-10
    94/100 stars
    		  Buy from Supplier
    4◦c  (ABclonal Biotechnology)
    97
    ABclonal Biotechnology 4◦c
    4◦C, supplied by ABclonal Biotechnology, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/claudin+4/Claudin+1+Rabbit+pAb/10__1002_slash_fft2__70281-167-26-36
    Average 97 stars, based on 1 article reviews
    4◦c - by Bioz Stars, 2026-10
    97/100 stars
    		  Buy from Supplier
    claudin 4  (ABclonal Biotechnology)
    94
    ABclonal Biotechnology claudin 4
    (A) Differential expression of IQGAP1 and claudins in normal and RCC tumors. Plots produced from pair‐wise gene expression correlation analysis using TPM data acquired from normal ( n = 72) and RCC tumor ( n = 523) samples between IQGAP1 and each of (top to bottom) claudin‐2 ( Normal : R = −0.4, p ≤ 0.00059; Tumor : R = 0.11, p ≤ 0.0089), <t>claudin</t> <t>4</t> ( Normal : R = 0.31, p ≤ 0.0081; Tumor : R = 0.032; p ≤ 0.46), and claudin 8 ( Normal : R = 0.36, p ≤ 0.0019; Tumor : R = −0.11, p ≤ 0.014). Pearson coefficient was used for calculating correlation. Results are plotted using a log scale and p ≤ 0.05 is considered significant. (B) IQGAP1 localizes to tubules in human kidneys and is mislocalized in RCC. Representative images of diverse IQGAP1 localization in normal human kidney tissues. Images are 20X showing localization in the distal tubules at the glomerulus brush border (upper) and at the medulla (lower). (C) Representative images of IQGAP1 and claudin‐4 localization in two different samples of renal cell carcinoma tissues: RCC5 (top panels) and RCC10 (lower panels). Scale bar = 20 mm. (D) Higher resolution images (×400) of IQGAP1 and claudin localization in RCC samples.
    Claudin 4, supplied by ABclonal Biotechnology, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/claudin+4/CLDN4+Rabbit+pAb/pmc13081790-59-20-22
    Average 94 stars, based on 1 article reviews
    claudin 4 - by Bioz Stars, 2026-10
    94/100 stars
    		  Buy from Supplier
    ab15102 claudin 4 rabbit  (Cusabio)
    94
    Cusabio ab15102 claudin 4 rabbit
    (A) Differential expression of IQGAP1 and claudins in normal and RCC tumors. Plots produced from pair‐wise gene expression correlation analysis using TPM data acquired from normal ( n = 72) and RCC tumor ( n = 523) samples between IQGAP1 and each of (top to bottom) claudin‐2 ( Normal : R = −0.4, p ≤ 0.00059; Tumor : R = 0.11, p ≤ 0.0089), <t>claudin</t> <t>4</t> ( Normal : R = 0.31, p ≤ 0.0081; Tumor : R = 0.032; p ≤ 0.46), and claudin 8 ( Normal : R = 0.36, p ≤ 0.0019; Tumor : R = −0.11, p ≤ 0.014). Pearson coefficient was used for calculating correlation. Results are plotted using a log scale and p ≤ 0.05 is considered significant. (B) IQGAP1 localizes to tubules in human kidneys and is mislocalized in RCC. Representative images of diverse IQGAP1 localization in normal human kidney tissues. Images are 20X showing localization in the distal tubules at the glomerulus brush border (upper) and at the medulla (lower). (C) Representative images of IQGAP1 and claudin‐4 localization in two different samples of renal cell carcinoma tissues: RCC5 (top panels) and RCC10 (lower panels). Scale bar = 20 mm. (D) Higher resolution images (×400) of IQGAP1 and claudin localization in RCC samples.
    Ab15102 Claudin 4 Rabbit, supplied by Cusabio, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/claudin+4/Rabbit+anti-+CLDN4+Polyclonal+Antibody/pm41930611-90-14-20
    Average 94 stars, based on 1 article reviews
    ab15102 claudin 4 rabbit - by Bioz Stars, 2026-10
    94/100 stars
    		  Buy from Supplier
    claudin 4  (Proteintech)
    99
    Proteintech claudin 4
    (A) Differential expression of IQGAP1 and claudins in normal and RCC tumors. Plots produced from pair‐wise gene expression correlation analysis using TPM data acquired from normal ( n = 72) and RCC tumor ( n = 523) samples between IQGAP1 and each of (top to bottom) claudin‐2 ( Normal : R = −0.4, p ≤ 0.00059; Tumor : R = 0.11, p ≤ 0.0089), <t>claudin</t> <t>4</t> ( Normal : R = 0.31, p ≤ 0.0081; Tumor : R = 0.032; p ≤ 0.46), and claudin 8 ( Normal : R = 0.36, p ≤ 0.0019; Tumor : R = −0.11, p ≤ 0.014). Pearson coefficient was used for calculating correlation. Results are plotted using a log scale and p ≤ 0.05 is considered significant. (B) IQGAP1 localizes to tubules in human kidneys and is mislocalized in RCC. Representative images of diverse IQGAP1 localization in normal human kidney tissues. Images are 20X showing localization in the distal tubules at the glomerulus brush border (upper) and at the medulla (lower). (C) Representative images of IQGAP1 and claudin‐4 localization in two different samples of renal cell carcinoma tissues: RCC5 (top panels) and RCC10 (lower panels). Scale bar = 20 mm. (D) Higher resolution images (×400) of IQGAP1 and claudin localization in RCC samples.
    Claudin 4, supplied by Proteintech, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/claudin+4/CLDN7+Fusion+Protein/pm41932000-61-5-13
    Average 99 stars, based on 1 article reviews
    claudin 4 - by Bioz Stars, 2026-10
    99/100 stars
    		  Buy from Supplier
    anti claudin-4  (Proteintech)
    94
    Proteintech anti claudin-4
    (A) Differential expression of IQGAP1 and claudins in normal and RCC tumors. Plots produced from pair‐wise gene expression correlation analysis using TPM data acquired from normal ( n = 72) and RCC tumor ( n = 523) samples between IQGAP1 and each of (top to bottom) claudin‐2 ( Normal : R = −0.4, p ≤ 0.00059; Tumor : R = 0.11, p ≤ 0.0089), <t>claudin</t> <t>4</t> ( Normal : R = 0.31, p ≤ 0.0081; Tumor : R = 0.032; p ≤ 0.46), and claudin 8 ( Normal : R = 0.36, p ≤ 0.0019; Tumor : R = −0.11, p ≤ 0.014). Pearson coefficient was used for calculating correlation. Results are plotted using a log scale and p ≤ 0.05 is considered significant. (B) IQGAP1 localizes to tubules in human kidneys and is mislocalized in RCC. Representative images of diverse IQGAP1 localization in normal human kidney tissues. Images are 20X showing localization in the distal tubules at the glomerulus brush border (upper) and at the medulla (lower). (C) Representative images of IQGAP1 and claudin‐4 localization in two different samples of renal cell carcinoma tissues: RCC5 (top panels) and RCC10 (lower panels). Scale bar = 20 mm. (D) Higher resolution images (×400) of IQGAP1 and claudin localization in RCC samples.
    Anti Claudin 4, supplied by Proteintech, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/claudin+4/Claudin+4-specific+Antibody/pm41825712-72-0-19
    Average 94 stars, based on 1 article reviews
    anti claudin-4 - by Bioz Stars, 2026-10
    94/100 stars
    		  Buy from Supplier
    anti claudin4  (Proteintech)
    94
    Proteintech anti claudin4
    (A) Differential expression of IQGAP1 and claudins in normal and RCC tumors. Plots produced from pair‐wise gene expression correlation analysis using TPM data acquired from normal ( n = 72) and RCC tumor ( n = 523) samples between IQGAP1 and each of (top to bottom) claudin‐2 ( Normal : R = −0.4, p ≤ 0.00059; Tumor : R = 0.11, p ≤ 0.0089), <t>claudin</t> <t>4</t> ( Normal : R = 0.31, p ≤ 0.0081; Tumor : R = 0.032; p ≤ 0.46), and claudin 8 ( Normal : R = 0.36, p ≤ 0.0019; Tumor : R = −0.11, p ≤ 0.014). Pearson coefficient was used for calculating correlation. Results are plotted using a log scale and p ≤ 0.05 is considered significant. (B) IQGAP1 localizes to tubules in human kidneys and is mislocalized in RCC. Representative images of diverse IQGAP1 localization in normal human kidney tissues. Images are 20X showing localization in the distal tubules at the glomerulus brush border (upper) and at the medulla (lower). (C) Representative images of IQGAP1 and claudin‐4 localization in two different samples of renal cell carcinoma tissues: RCC5 (top panels) and RCC10 (lower panels). Scale bar = 20 mm. (D) Higher resolution images (×400) of IQGAP1 and claudin localization in RCC samples.
    Anti Claudin4, supplied by Proteintech, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/claudin+4/Claudin+4-specific+Antibody/bio_rxiv__64898__2026__03__09__709900-56-33-34
    Average 94 stars, based on 1 article reviews
    anti claudin4 - by Bioz Stars, 2026-10
    94/100 stars
    		  Buy from Supplier

    Image Search Results


    (A) Differential expression of IQGAP1 and claudins in normal and RCC tumors. Plots produced from pair‐wise gene expression correlation analysis using TPM data acquired from normal ( n = 72) and RCC tumor ( n = 523) samples between IQGAP1 and each of (top to bottom) claudin‐2 ( Normal : R = −0.4, p ≤ 0.00059; Tumor : R = 0.11, p ≤ 0.0089), claudin 4 ( Normal : R = 0.31, p ≤ 0.0081; Tumor : R = 0.032; p ≤ 0.46), and claudin 8 ( Normal : R = 0.36, p ≤ 0.0019; Tumor : R = −0.11, p ≤ 0.014). Pearson coefficient was used for calculating correlation. Results are plotted using a log scale and p ≤ 0.05 is considered significant. (B) IQGAP1 localizes to tubules in human kidneys and is mislocalized in RCC. Representative images of diverse IQGAP1 localization in normal human kidney tissues. Images are 20X showing localization in the distal tubules at the glomerulus brush border (upper) and at the medulla (lower). (C) Representative images of IQGAP1 and claudin‐4 localization in two different samples of renal cell carcinoma tissues: RCC5 (top panels) and RCC10 (lower panels). Scale bar = 20 mm. (D) Higher resolution images (×400) of IQGAP1 and claudin localization in RCC samples.

    Journal: Clinical and Translational Science

    Article Title: The IQGAP1 ‐Claudin4‐ JNK Signaling Axis as a Differential Biomarker in Renal Cell Carcinoma

    doi: 10.1111/cts.70539

    Figure Lengend Snippet: (A) Differential expression of IQGAP1 and claudins in normal and RCC tumors. Plots produced from pair‐wise gene expression correlation analysis using TPM data acquired from normal ( n = 72) and RCC tumor ( n = 523) samples between IQGAP1 and each of (top to bottom) claudin‐2 ( Normal : R = −0.4, p ≤ 0.00059; Tumor : R = 0.11, p ≤ 0.0089), claudin 4 ( Normal : R = 0.31, p ≤ 0.0081; Tumor : R = 0.032; p ≤ 0.46), and claudin 8 ( Normal : R = 0.36, p ≤ 0.0019; Tumor : R = −0.11, p ≤ 0.014). Pearson coefficient was used for calculating correlation. Results are plotted using a log scale and p ≤ 0.05 is considered significant. (B) IQGAP1 localizes to tubules in human kidneys and is mislocalized in RCC. Representative images of diverse IQGAP1 localization in normal human kidney tissues. Images are 20X showing localization in the distal tubules at the glomerulus brush border (upper) and at the medulla (lower). (C) Representative images of IQGAP1 and claudin‐4 localization in two different samples of renal cell carcinoma tissues: RCC5 (top panels) and RCC10 (lower panels). Scale bar = 20 mm. (D) Higher resolution images (×400) of IQGAP1 and claudin localization in RCC samples.

    Article Snippet: Slides were stained overnight at 4°C with the following primary antibodies: IQGAP1 (Santa Cruz, Cat. No. sc‐1079, 1:200 dilution) and claudin‐4 (1:200, Ab clonal).

    Techniques: Quantitative Proteomics, Produced, Gene Expression